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MCW: SARGEANT HEALTH CENTER Research Technologist I in MILWAUKEE, Wisconsin

JOB REQUIREMENTS: Position Description: Research Technologist I Purpose A Research Technologist I position is immediately available in the E lab at the Neuroscience Research Center at the Medical College of Wisconsin. The lab investigates non-neural mechanisms of neurodegeneration in normal aging, as well as genetic underpinnings of Alzheimer\'s disease-related neurodegeneration, primarily using C. elegans (roundworm) as a model system. The lab is seeking a motivated Research Technologist to provide support for lab research through ordering and maintaining supplies, preparing reagents/media, maintaining the biological material database/libraries, and managing general lab maintenance. Under the direction and supervision of the principal investigator, the Research Technologist is also expected to execute experiments/assays to generate data for research projects/publications/grant applications. While experience in molecular biology, molecular genetics, microscopy, and/or working with C. elegans is welcome, hands-on training will be available for all required tasks. Demonstrated proficiency in basic biology/chemistry lab techniques (weighing, pipetting, making solutions/buffers, dilutions, centrifugation, etc.) is required. Individuals with experience in molecular biology (standard PCR, qPCR, plasmid design and construction) are encouraged to apply. Primary Functions * Prepare sterile buffers, media, reagents, nematode culture (nutrient agar) plates and competent cells for transformation etc. * Execute assigned duties for ordering and maintaining the supply of inventories. * Manage and organize the C. elegans strain/plasmid/primers/RNAi library collection in the lab. * Perform and assist in assigned assays, including but not limited to PCR, qPCR, worm picking, worm genotyping, molecular cloning, microinjection to generate transgenic animals, in vivo imaging using widefield and confocal fluorescence microscopy, and quantification of neurodegeneration phenotypes. * Record and compile accurate records and communicate summaries of experimental results. * Assist with the evaluation, interpretation and assessment of experimental data and quality control of assays. * Attend lab meetings with principal investigator, graduate students, research staff, participate in discussion, and present data as needed. * Maintain lab records and uphold safety regulations. * Perform routine maintenance on specialized equipment used in the lab. Communicate with vendors for troubleshooting in case of malfunctioning. * Oversee daily lab operation in the absence of the principal investigator. * Will have opportunities to contribute to... For full info follow application link. EEO/AA Minorities/Females/Vets/Disability The Medical College of Wisconsin is an affirmative action/equal opportunity employer and does not discriminate in hiring or employment on the basis of age, sex, race, color, religion, national origin, veteran status, disability or sexual orientation. ***** APPLICATION INSTRUCTIONS: Apply Online: ipc.us/t/57287F9C075845C1 Qualified females, minorities, and special disabled veterans and other veterans are encouraged to apply.

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