Job Information
Johns Hopkins University Research Specialist II in Baltimore, Maryland
The Coyne laboratory at the Johns Hopkins Department of Neurology and Brain Science Institute seeks a Research Specialist II to join our laboratory studying mechanisms and treatment of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). We use a combination of cellular, molecular, and biochemical techniques in cultured induced pluripotent stem cell derived neurons, transgenic mice, Drosophila, and postmortem tissue to study the disruption of ESCRT-III nuclear surveillance and nuclear pore complexes and identify novel therapeutic strategies. We are seeking an experienced laboratory specialist to become an integral part of our group, supporting and carrying out collaborative and independent work at the bench, participating in data analysis and research objectives, and supporting the day-to-day functioning of the laboratory.
Specific Duties and Responsibilities
Experimental techniques and areas may include mouse husbandry, genotyping, perfusion and dissection, and tissue sectioning, Drosophila husbandry, iPSC cell culture (passaging, maintenance, neuronal differentiation, cryopreservation, and transfection), immunofluorescence, protein and RNA extraction, Western blotting, qPCR, and microscopy.
General laboratory support may include ordering materials and supplies, monitoring laboratory inventory, managing and maintaining basic laboratory equipment, and preparing common solutions and reagents.
Follow established protocols and use independent judgement in conducting experiments and evaluating the next course of action.
Effectively work in both an individual and team setting including training and supporting students in general laboratory tasks.
Maintain excellent written records and electronic databases, using routine software applications, such as e-mail and Microsoft Office (Word, Excel, PowerPoint), as well as scientific software including statistical/graphing programs (Prism), image analysis (MetaXpress, Imaris, ImageJ) and maintenance of an electronic lab notebook (OneNote, DropBox).
Use universal safety precautions to protect self and co-workers from biohazardous materials, including blood-borne pathogens.
Complete annual university biohazard/universal precaution/radiation safety training, as appropriate.
Monitor biohazard/radiation safety standards and proper handling of potentially hazardous chemical and biological agents and/or radiation sources in the workplace.
Perform miscellaneous related duties as assigned
Minimum Qualifications
Bachelor's Degree in Biology, Chemistry or related field.
Two years of laboratory experience performing relevant techniques.
Master's degree, with related graduate research, may substitute for experience.
Additional education may substitute for required experience and additional related experience may substitute for required education beyond HS Diploma/Graduation Equivalent, to the extent permitted by the JHU equivalency formula.
Preferred Qualifications
A minimum of two years of laboratory experience with mouse handling and genotyping OR.
A minimum of three years of laboratory experience in cellular/molecular biology techniques and cell culture with a variety of cell types, including neurons.
Classified Title: Research Specialist II
Role/Level/Range: ACRP/03/MB
Starting Salary Range: $41,300 - $72,300 Annually ($48,000 targeted; Commensurate w/exp.)
Employee group: Full Time
Schedule: M-F/8:30am-5pm
FLSA Status: Exempt
Location: School of Medicine Campus
Department name: Neurodegenerative and Injury Program
Personnel area: School of Medicine
The listed salary range represents the minimum and maximum Johns Hopkins University offers for this position, based on a good faith estimate at the time of posting. Actual compensation will vary depending on factors such as location, skills, experience, market conditions, education, and internal equity. Not all candidates will qualify for the highest salary in the range.
Johns Hopkins provides a comprehensive benefits package supporting health, career, and retirement. Learn more: https://hr.jhu.edu/benefits-worklife/.
Equal Opportunity Employer
All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.
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